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首页 > 中国极地考察知识库 > 成果信息库 > 已审查 快速升温后极地甲藻Polarella glacialis的生理响应

快速升温后极地甲藻Polarella glacialis的生理响应

【标题】快速升温后极地甲藻Polarella glacialis的生理响应

【Title】PHYSIOLOGICAL RESPONSE OFPOLARELLA GLACIALIS, A POLAR DINOFLAGELLATE, TO TEMPERATURE RISES

【DOI】10.3724/SP.J.1084.2013.00061

【作者】 郑淑娴; 何剑锋; 王桂忠; 林森杰; 张芳

【Author】 Zheng Shuxian ; He Jianfeng ; Wang Guizhong ; Lin Senjie ; Zhang Fang

【期刊】极地研究

【Journal】ADVANCES IN POLAR SCIENCE

【期刊年份】2013

【卷】25

【期】1

【关键词】 Polarella glacialis; 细胞周期; Rubisco; PCNA

【Keywords】 Polarella glacialis; cell cycle; Rubisco; PCNA

【摘要】 Polarella glacialis最早发现于南极固定冰和北冰洋水层,最近在温带海域也发现其相关基因。专抗甲藻的Rubisco、PCNA抗体在该藻细胞内各检测到~53 kDa和~55 kDa的特异性条带。对该藻的梯度温度培养实验显示,4℃生长的P. glacialis细胞密度为1.1×105 cells/ml时仍能继续增殖,一天中藻细胞内Rubisco的表达量基本保持恒定,PCNA的表达量峰值与%S峰值相对应,当温度迅速上升至15、20℃时,藻株处于胁迫状态,细胞密度迅速减少,正常的细胞周期被干扰,大多数细胞分裂活动不活跃或停止分裂,Rubisco、PCNA的表达量大幅减少甚至消失,昼夜表达特征改变,其中20℃对藻株的胁迫作用更加显著。15℃培养下的P. glacialis细胞密度并不立即减少,细胞周期与两种指示蛋白表达特征显示仍有部分细胞完成分裂。本研究为该藻的相关基因在温带水域的出现提供了可能的解释,并推测在相对长期渐进的极地增温过程中,P. glacialis可能继续存在。

【Abstract】orella glacialiswas discovered from the Antarctic land-fast sea ice and Arctic water column. Recently,P. glacialis-like genotypes were found in temperate waters. The dinoflagellate Rubisco and PCNA antibodies detected specific bands of ~53kDa and ~55kDa inP. glacialis. As shown by the gradient temperature experiment, the diel expression of Rubisco protein maintained nearly stable in the 4℃ culture. The diel expression of PCNA closely related with the %S peak.P. glacialiscontinued to grow after the cell density increased to 1.1×105cells.ml-1. When the temperature rose up to 15 and 20℃, the dinoflagellate was stressed with fast reducing cell density, altered cell cycle, inactive cell division or even stopped, and greatly reduced abundances and altered diel expression patterns of Rubisco and PCNA. The culture was stressed by 20℃ more. However, the cell density ofP. glacialisdid not quickly decrease when moved to 15℃. And the cell cycle pattern and the reserved expressing rhythm of the indicative proteins indicated possible cell division of a small part of cells. We concluded that as a polar dinoflagellate,P. glacialismight exist for a long time in the relatively long and gradual process of warming in the polar region. And it was not surprised for the presence of theP. glacialis-like genotypes in temperate waters.

【基金/项目】 南、北极海洋微微型浮游植物群落及其生态作用研究 ; 北极高纬海域混合营养鞭毛类及其生态作用研究

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