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Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

【标题】Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

【Title】Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

【DOI】10.1007/s13131-013-0271-y

【作者】 俞勇; LI Huirong; 曾胤新; 何剑锋; LEE SangH; 孙鲲

【Author】 Yu Yong ; Huirong Li ; Zeng Yinxin ; He Jianfeng ; LEE SangH ; Sun Kun

【期刊】中国海洋学报

【Journal】Acta Oceanologica Sinica

【期刊年份】2013

【卷】32

【期】6

【关键词】 diversity, planktonic bacteria, 16S rRNA gene clone library, Chukchi Borderland

【Keywords】 diversity, planktonic bacteria, 16S rRNA gene clone library, Chukchi Borderland

【摘要】Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes,Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries.SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammaproteobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell intoGammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. Themost common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFracdata showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.

【Abstract】Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes,Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries.SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammaproteobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell intoGammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. Themost common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFracdata showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.

【基金/项目】 极地海洋上层生态系统中玫瑰杆菌支系的多样性及生态功能分析 ; 北极细菌的DMSP代谢途径初步分析 ; 北极海洋生态系统对海冰快速变化响应的评价技术 ; 站基生物生态环境本底考察 ; the National High-Tech Research and Development Programof China under contract No. 2012AA021706

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