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Archaeal diversity and abundance within different layers of summer sea-ice and seawater from Prydz Bay, Antarctica

【标题】Archaeal diversity and abundance within different layers of summer sea-ice and seawater from Prydz Bay, Antarctica

【Title】Archaeal diversity and abundance within different layers of summer sea-ice and seawater from Prydz Bay, Antarctica

【作者】 MA Jifei; DU Zongjun; 罗玮; 俞勇; 曾胤新; 陈波; 李会荣

【Author】 MA Jifei ; DU Zongjun ; Luo Wei ; Yu Yong ; Zeng Yinxin ; Chen Bo ; Li Kuairong

【期刊】极地研究

【Journal】ADVANCES IN POLAR SCIENCE

【期刊年份】2014

【卷】25

【期】1

【关键词】 Antarctica summer sea-ice archaea diversity abundance

【Keywords】 Antarctica summer sea-ice archaea diversity abundance

【摘要】Fluorescence in-situ hybridization (FISH) and 16S rRNA gene clone library analyses were used to determine the abundance and diversity of archaea in Prydz Bay, Antarctica. Correlation analysis was also performed to assess links between physicochemical parameters and archaeal abundance and diversity within the sea-ice. Samples of sea-ice and seawater were collected during the 26th Chinese National Antarctic Research Expedition. The results of FISH showed that archaea were relatively abundant within the top layer of the sea-ice, and correlation analysis suggested that the concentration of NH4+ might be one of the main factors underlying this distribution pattern. However, using 16S rRNA gene libraries, archaea were not detected in the top and middle layers of the sea-ice. All archaeal clones obtained from the bottom layer of the sea-ice were grouped into the Marine Group I Crenarchaeota while the archaeal clones from seawater were assigned to Marine Group I Crenarchaeota, Marine Group II Euryarchaeota, and Marine Group III Euryarchaeota. Overall, the findings of this study showed that the diversity of archaea in the sea-ice in Prydz Bay was low.

【Abstract】Fluorescence in-situ hybridization (FISH) and 16S rRNA gene clone library analyses were used to determine the abundance and diversity of archaea in Prydz Bay, Antarctica. Correlation analysis was also performed to assess links between physicochemical parameters and archaeal abundance and diversity within the sea-ice. Samples of sea-ice and seawater were collected during the 26th Chinese National Antarctic Research Expedition. The results of FISH showed that archaea were relatively abundant within the top layer of the sea-ice, and correlation analysis suggested that the concentration of NH4+ might be one of the main factors underlying this distribution pattern. However, using 16S rRNA gene libraries, archaea were not detected in the top and middle layers of the sea-ice. All archaeal clones obtained from the bottom layer of the sea-ice were grouped into the Marine Group I Crenarchaeota while the archaeal clones from seawater were assigned to Marine Group I Crenarchaeota, Marine Group II Euryarchaeota, and Marine Group III Euryarchaeota. Overall, the findings of this study showed that the diversity of archaea in the sea-ice in Prydz Bay was low.

【基金/项目】 南极普里兹湾海冰中氨氧化细菌、氨氧化古菌多样性及其生态功能分析 ; 极地海洋上层生态系统中玫瑰杆菌支系的多样性及生态功能分析 ; 南极菲尔德斯半岛附近海域表层海水氨氧化古菌多样性及其富集培养 ; 站基生物生态环境本底考察

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