【Title】DIVERSITY ANALYSIS OF FUNGI ISOLATED FROM NY-ÅLESUND, SVALBARD, AND SCREENING FOR BIOTRANSFORMATION OF GENTIOPICROSIDE
许丹 马吉飞 苗祯 李会荣 陈波
Xu Dan, Ma Jifei, Miao Zhen, Li Huirong, Chen Bo
biotransformation of gentiopicroside
【摘要】 采用多种培养基对北极高纬地区（土壤及海洋沉积物）真菌进行分离纯化，共得真菌65株。通过分析菌株的18S rDNA序列研究了它们的系统发育多样性，18S rDNA序列分析表明，65株真菌分属25个属，其中数量上位居第一的是青霉属（Penicillium），有菌株20株，其次为丛赤壳属（Nectria）和节枝孢属（Articulospora），各有菌株6株。同时对这65株真菌开展转化龙胆苦苷阳性菌株的筛选。以β-葡萄糖苷酶作为筛选转化菌株的标志酶于65株真菌中筛选到阳性菌株28株，其中产酶能力强的9株菌作为转化实验的备选菌株，以龙胆苦苷为底物进行转化，将转化粗提物进行TLC及HPLC分析，最终确定将C-5作为转化龙胆苦苷的菌株。根据菌株形态特征和核酸序列分析结果，初步鉴定菌株C-5为青霉属真菌。暂定名为Penicillium sp.C-5。
65 strains of fungi isolated from the arctic high-latitude soil and marine sediments were investigated. Their phylogenetic diversity were analyzed by their 18S rDNA sequencing. Classification of 65 fungi strains based on 18S rDNA gene showed that these fungi represented 26 genera, of which the number of Penicillium was the most of 19 strains ,followed by Nectria and Articulospora ,with 6 strains respectively.The strains which can transform gentiopicroside were screened. β-glucosidase was chosen as marker enzyme to screen the positive strains. And 28 strains were tested to produce it. Among them, 9 strains were selected to transform gentiopicroside. And the crude extracts of them ware tested by TLC and HPLC. Ultimately strain C-5 was determined as the biotransformation strain. According to the morphological features and nucleic acid sequences analysis, strain C-5 was identified for the genus of Penicillium. It was tentatively named as Penicillium sp.C-5.