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Extracellular enzymes of cold-adapted bacteria from Arctic sea ice, Canada Basin

【标题】Extracellular enzymes of cold-adapted bacteria from Arctic sea ice, Canada Basin

【Title】Extracellular enzymes of cold-adapted bacteria from Arctic sea ice, Canada Basin

【DOI】10.1007/s00300-009-0654-x

【作者】 俞勇; 李会荣; 曾胤新; 陈波

【Author】 Yu Yong ; Li Kuairong ; Zeng Yinxin ; Chen Bo

【期刊】Polar Biology

【Journal】Polar Biology

【期刊年份】2009

【卷】32

【期】10

【关键词】 Extracellular enzymes; Psychrophilic; Psychrotolerant; Cold-active lipase; Sea ice; Arctic

【Keywords】 Extracellular enzymes; Psychrophilic; Psychrotolerant; Cold-active lipase; Sea ice; Arctic

【摘要】A total of 338 aerobic heterotrophic bacterial strains were isolated from Arctic sea ice, Canada Basin (77°30′N–80°12′N). The capability of the isolates to produce protease, lipase, amylase, chitinase, β-galactosidase, cellulase and/or agarase was investigated. Isolates that were able to degrade tributyrin, skim milk, starch, lactose and chitin accounted for 71.6, 65.7, 38.5, 31.6 and 16.9% of sea ice strains, respectively. Lipase producers and/or protease producers were phylogenetically widespread among the isolated strains. Starch and/or lactose hydrolytic strains were mainly distributed among Colwellia, Marinomonas, Pseudoalteromonas, Pseudomonas and Shewanella isolates. Pseudoalteromonas tetraodonis, Pseudoalteromonas elyakovii, Bacillus firmus and Janibacter melonis isolates all have the ability to degrade chitin. Only some strains belonging to Pseudoalteromonas genus scored positive for agarase (6) and cellulose (9). The temperature dependences for lipase activities were determined for five psychrophilic and six psychrotolerant bacteria. At low temperatures, the psychrophilic bacterial lipase activity was not significantly higher than psychrotolerant bacterial lipase, though all lipases showed remarkably high activity with 10–36% residual activity at 0°C.

【Abstract】A total of 338 aerobic heterotrophic bacterial strains were isolated from Arctic sea ice, Canada Basin (77°30′N–80°12′N). The capability of the isolates to produce protease, lipase, amylase, chitinase, β-galactosidase, cellulase and/or agarase was investigated. Isolates that were able to degrade tributyrin, skim milk, starch, lactose and chitin accounted for 71.6, 65.7, 38.5, 31.6 and 16.9% of sea ice strains, respectively. Lipase producers and/or protease producers were phylogenetically widespread among the isolated strains. Starch and/or lactose hydrolytic strains were mainly distributed among Colwellia, Marinomonas, Pseudoalteromonas, Pseudomonas and Shewanella isolates. Pseudoalteromonas tetraodonis, Pseudoalteromonas elyakovii, Bacillus firmus and Janibacter melonis isolates all have the ability to degrade chitin. Only some strains belonging to Pseudoalteromonas genus scored positive for agarase (6) and cellulose (9). The temperature dependences for lipase activities were determined for five psychrophilic and six psychrotolerant bacteria. At low temperatures, the psychrophilic bacterial lipase activity was not significantly higher than psychrotolerant bacterial lipase, though all lipases showed remarkably high activity with 10–36% residual activity at 0°C.

【基金/项目】 南极主要海洋生物资源和生态过程监测与评估技术研究 ; 极端微生物及其功能利用的基础研究 ; 极地环境嗜冷菌新种的发现与分类研究 ; 产蛋白酶极地海洋浮游细菌的系统发育多样性比较及其生理生态特征分析 ; 海洋微生物菌种资源整理整合与共享试点

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