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首页 > 中国极地考察知识库 > 成果信息库 > 已审查 UV-B短期照射对北极冰川小球藻Chlorella sp.的生物学效应

UV-B短期照射对北极冰川小球藻Chlorella sp.的生物学效应

【标题】UV-B短期照射对北极冰川小球藻Chlorella sp.的生物学效应

【Title】The physiological and biochemical responses of an Arctic microalgae (Chlorella sp.) to UV-B radiation

【作者】 彭小伟; 何剑锋; 罗玮; 李运广; 蔡明红

【Author】

【期刊】极地研究

【Journal】

【期刊年份】2010

【卷】Vol. 22

【期】

【关键词】 UV-B辐射 小球藻 生化组分

【Keywords】 UV-B radiation, Chlorella sp., biochemical composition

【摘要】以一种分离自北极冰川融水的淡水微藻(Chlorella sp.)为实验材料,设置两个不同强度UV-B(45μw/cm2、76μw/cm2)照射处理、每4小时取样检测,对其生化组分进行分析,了解微藻对UV-B辐射的响应。研究结果显示小球藻经辐射处理后,其生化成分发生变化:(1)在较低强度的UV-B(45μw/cm2)辐射下,叶绿素a(Chla)和类胡萝卜素(Car)含量先升后降,且在4h时有最大值;高强度UV-B(76μw/cm2)辐射下,Chla和Car含量随辐射时间延长基本呈下降趋势。(2)超氧阴离子(O2?ˉ)和丙二醛(MDA)含量基本随辐照时间的延长和辐照强度的增大提高,过氧化氢(H2O2)含量变化虽随辐照时间的延长呈上升趋势,但直到12h是才表现为明显的提高。(3)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性在低UV-B处理下,随辐照时间的延长呈升高趋势;在高UV-B辐射下,SOD活性先升后降,CAT活性上下波动。(4)UV-B辐射使小球藻脂肪酸组成发生了变化。这些结果表明:UV-B,尤其是高强度或较高剂量的UV-B会对北极冰川微藻带来不利的影响。

【Abstract】The response of an Arctic microalgae strain (Chlorella sp.) to the enhancement of UV-B radiation was explored in this paper. The effects of three different intensity (0 μw/cm2, 45 μw/cm2, 76 μw/cm2 ) within 12 h radiation following with 4 h non-radiation recovery were studied, while the biochemical components were tested by sampling every 4 h. Our results showed that: (1) Under the lower intensity of UV-B (45μw/cm2) radiation, Chla and Car content increased in the beginning 4 h and then decreased. The maximum value presented after 4 hrs. Meanwhile, under high-intensity UV-B (76μw/cm2) radiation, Chla and CAR content constantly decreased. (2) The content of O2?ˉ and MDA enhanced along with radiation time and intensity. Moreover, H2O2 content also increased with irradiation time; significant vary happened after 8 h radiation. (3) SOD, CAT activity under the lower UV-B treatment, increased with irradiation time as well. Under higher UV-B radiation, SOD activity increased at the beginning 4 hrs and then decreased constantly, CAT activity fluctuated from time to time.(4)The fatty acid composition of Chlorella was influenced. The results supported that UV-B had adverse effects on the Arctic glaciers microalgae, and the effect of hurt was more significant under high-intensity or high doses of UV-B.

【基金/项目】

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