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Spatial heterogeneity of a microbial community in Kongsfjorden, Svalbard during late summer 2006 and its relationship to biotic and abiotic factors

【标题】Spatial heterogeneity of a microbial community in Kongsfjorden, Svalbard during late summer 2006 and its relationship to biotic and abiotic factors

【Title】Spatial heterogeneity of a microbial community in Kongsfjorden, Svalbard during late summer 2006 and its relationship to biotic and abiotic factors

【作者】 郭超颖; 何剑锋; 张芳; 蔡明红; 王桂忠

【Author】 Guo Chaoying ; He Jianfeng ; Zhang Fang ; Cai Minghong ;

【期刊】极地研究

【Journal】

【期刊年份】2011

【卷】22

【期】

【关键词】 Microbial community; Genetic diversity; Kongsfjorden; Arctic fjord

【Keywords】 Microbial community; Genetic diversity; Kongsfjorden; Arctic fjord

【摘要】The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments (temperature, salinity, silicate, phosphate and nitrate, p <0.05) of the inner and outer basins of Kongsfjorden (Spitsbergen, Arctic) were studied using polymerase chain reaction-denaturing gradient gel electrophoresis(DGGE) fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria:Proteobacteria (Alpha, Beta and Gamma), Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m (outer fjord) was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.

【Abstract】The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments (temperature, salinity, silicate, phosphate and nitrate, p <0.05) of the inner and outer basins of Kongsfjorden (Spitsbergen, Arctic) were studied using polymerase chain reaction-denaturing gradient gel electrophoresis(DGGE) fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria:Proteobacteria (Alpha, Beta and Gamma), Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m (outer fjord) was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.

【基金/项目】

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