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Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

【标题】Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

【Title】Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

【作者】 刘晓辉; 俞勇; 李会荣; 张琳; 蒋欣音; 任大明

【Author】 Li Xiaohui ; Yu Yong ; Li Kuairong ; Zhang Lin ; Jiang Xinyin ; Ren Daming

【期刊】极地研究

【Journal】

【期刊年份】2008

【卷】19

【期】1

【关键词】 Arctic sea ice microorganisms; culture condition; Pseudoalteronomas; hydrolase

【Keywords】

【摘要】Arctic sea ice in the polar region provides a cold habitat for microbial community.Arctic sea ice microorganisms are revealed to be of considerable importance in basic research and potential in biotechnological application. This paper investigated the culture condition and extracellular hydrolase of 14 strains of different Arctic sea ice bacteria. The results showed that optimal growth temperature of strains is 15℃or 20℃. The optimal pH is about 8.0. They hardly grow at acid condition. 3% NaCl is necessary for better growth. These strains have different abilities in producing amylase, protcase, cellulase and lipase. Pseudoalteronomas sp. Bsi429 and Pseudoalteronomas sp. Bsi539 produced both cellulose, protease and lipase. These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources.

【Abstract】Arctic sea ice in the polar region provides a cold habitat for microbial community.Arctic sea ice microorganisms are revealed to be of considerable importance in basic research and potential in biotechnological application. This paper investigated the culture condition and extracellular hydrolase of 14 strains of different Arctic sea ice bacteria. The results showed that optimal growth temperature of strains is 15℃or 20℃. The optimal pH is about 8.0. They hardly grow at acid condition. 3% NaCl is necessary for better growth. These strains have different abilities in producing amylase, protcase, cellulase and lipase. Pseudoalteronomas sp. Bsi429 and Pseudoalteronomas sp. Bsi539 produced both cellulose, protease and lipase. These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources.

【基金/项目】

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